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. 2005 Jan;16(1):141–152. doi: 10.1091/mbc.E04-08-0703

Figure 7.

Figure 7.

Spc29p-YFP and Spc42p-YFP do not localize to the aberrant structure in the spc110-226 mutant. (A) Localization of Spc29p-YFP was determined in cells containing Spc97p-CFP after incubation at the nonpermissive temperature for 225 min (MAMY2). Spc29p-YFP localizes to two foci and is absent from the third foci in 100% of the cells, N = 174. (B) Localization of Spc42p-YFP was determined in cells containing Spc97p-CFP after incubation at the nonpermissive temperature for 225 min (MAMY3). Spc42p-YFP localizes to two foci and is absent from the third foci in 100% of the cells, N = 182. (C) Colocalization of the SPBs and/or fragments with the nuclear envelope was determined in cells containing Spc97p-Venus and Nic96p-CFP after incubation at the nonpermissive temperature for 225 min (TYY161). In 94% of cells with three SPBs and/or fragments, all three entities seemed to either colocalize with the nuclear envelope or lie within the nucleus, N = 64. Bar, 5 μm.