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KIF3 is not required for rab6-induced Golgi-to-ER transport. (A) HeLa cells were treated with KAP3 siRNA or control duplexes for 48 h and then lysed in sample buffer. Western Blot analysis was performed with antibodies to KAP3 and α-tubulin for normalization of protein levels. KAP3 RNAi interference specifically decreases KAP3. (B) KAP3-depleted cells show a typical redistribution of GalNAcT2 GFP to the ER, 7 h after injection of DNA encoding rab6-GTP (asterisks).
