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Anillin affinity chromatography. (A) Eluates from anillin (full length [FL] or without pleckstrin homology domain [-PH]), Polo kinase (PLX1), or GST columns. Left, cytostatic factor arrested extracts. Right, interphase extracts. (B) Eluates from GST and fulllength anillin columns immunoblotted with antibodies to nonmuscle myosin II heavy chain.
