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. 2005 Jan;16(1):202–211. doi: 10.1091/mbc.E04-08-0742

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Copyright © 2005, The American Society for Cell Biology

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Figure 5. — OPD as a function of organelle diameter (GV in oil). The OPD of a homogeneous organelle relative to the nucleoplasm should increase linearly with its thickness (t) in the z-axis. Because there is no easy way to measure the thickness, we assumed that organelles are spherical and plotted OPD as a function of diameter measured in the x,y plane. The data for each organelle were fitted to a straight line passing through the origin. The slope of each line (OPD/t) can be used to estimate the refractive index of the organelle.