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. 2005 Jan;16(1):218–230. doi: 10.1091/mbc.E04-07-0560

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Figure 2. — Influence of growth phase on glutathione levels in the parental strain (BY4743) and representative glutathione-overexcreting mutants. The parent (squares) and representative glutathione-overexcreting mutants vps27 (circles), ira2 (triangles), and doa1 (crosses) were inoculated (A₆₀₀ of 0.01) in SD medium and incubated at 30°C. (A) Growth (open symbols; A₆₀₀) and medium pH (closed symbols; initial pH of medium was 4.5) and (B) intracellular glutathione (closed symbols; μM/A₆₀₀) and extracellular glutathione (open symbols; μM/A₆₀₀) were quantified at intervals between 15 and 44 h postinoculation as indicated.