Figure 4.
Influence of the relative availability of BCAAs on extracellular glutathione accumulation by selected deletion mutants. The parent (BY4743) and deletion mutants shown were inoculated (initial A600 of 0.01) in SD medium supplemented with leucine, isoleucine, and valine: 1 time, 131, 66, and 59 mg l-1 (open bars); 2 times, 262, 112, and 118 mg l-1 (shaded bars); 4 times, 524, 264, and 236 mg l-1 (closed bars), respectively, and grown to stationary phase (2 d; 30°C) at which time extracellular glutathione was quantified.