Figure 1.
Schematic of the SERS assay principle and design of SSEA-5-conjugated and TRA-1-60-conjugated nanoparticles. (A) The assay principle using SSEA-5-conjugated and/or TRA-1-60-conjugated nanoparticles to detect pluripotent stem cells. Cells were incubated with SSEA-5- and/or TRA-1-60-conjugated nanoparticles. After washing, SERS signals from cell pellets were detected by laser and SERS spectra were analyzed. (B) Schematic diagram showing the preparation of Raman-encoded, PEG-stabilized, and SSEA-5-functionalized Au nanoparticles. (C) Preparation of Raman-encoded, PEG-stabilized, and TRA-1-60-functionalized Au nanoparticles.