Table 1.
Yeast two-hybrid analysis of the interaction of the PBD domain of PakB with human (Hs) and Dictyostelium Rho family GTPases
| Rho GTPase | Strength of interaction |
|---|---|
| Rac1a Q61L | +++ |
| Rac1b G12V | +++ |
| Rac1c G12V | +++ |
| RacA G12V | +++ |
| RacB G12V | +++ |
| RacC G15V | +++ |
| RacD G17V | - |
| RacE G20V | + |
| RacF1 G12V | +++ |
| RacG G12V | + |
| RacH M13V | - |
| RacI S14V | - |
| RacJ D18V | - |
| RacL G12V | - |
| HsRac1 G12V | +++ |
| HsCdc42 G12V | +++ |
| HsRhoA G14V | - |
The strength of the interaction was estimated by colony-lift β-galactosidase filter assays. Color development was monitored 4 days after plating and judged to be negative (-), weak (+), medium (++), or strong (+++).