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. 2017 Apr 12;37(15):4023–4031. doi: 10.1523/JNEUROSCI.3442-16.2017

Figure 3.

Figure 3.

Astrocytic LRP1 deletion in APP/PS1; aLrp1−/− mice. Astrocyte-specific LRP1-KO mice were generated by crossing the Lrp1flox/flox mice with GFAP-Cre mice, and further bred into an APP/PS1 background. A, Adult astrocytes were established from APP/PS1 and APP/PS1; aLrp1−/− mice (2–3 months of age). The astrocytes were costained with a rabbit anti-LRP1 antibody and a mouse anti-GFAP antibody. Scale bar, 50 μm. B, Brain LRP1 and apoE levels in the cortex (n = 9/group) and hippocampus (n = 6–10/group) of APP/PS1 and APP/PS1; aLrp1−/− mice were quantified by Western blot analysis. Data are mean ± SEM. **p < 0.01. C, Cortical and hippocampal slides from control APP/PS1 and APP/PS1; aLrp1−/− mice were costained with an LRP1 antibody and a neuronal marker anti-NeuN or an astrocyte marker anti-GFAP, respectively. Scale bar, 50 μm. The colocalization of the immunoreactivities of LRP1 and GFAP is abundant in sections from APP/PS1 mice but minimum in those from APP/PS1; aLrp1−/− mice. Colocalization with neuronal marker NeuN was not affected by astrocytic LRP1 deletion.