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. 2017 Apr 12;37(15):4023–4031. doi: 10.1523/JNEUROSCI.3442-16.2017

Figure 5.

Figure 5.

LRP1 deficiency in astrocytes impairs Aβ clearance but not Aβ production in vivo. A, B, Aβ metabolism in the hippocampus of APP/PS1 and APP/PS1; aLrp1−/− mice (n = 4/group) at the age of 3–4 months was analyzed by in vivo microdialysis. A, Baseline Aβ40 levels were measured. To assess Aβ40 half-life, the mice were treated with a γ-secretase inhibitor, and the hippocampal ISF Aβ40 concentrations were monitored. B, The common logarithm of percentage baseline ISF Aβ40 concentrations versus time was plotted. The mean ISF Aβ elimination half-life (t1/2) was calculated for each mouse as the slope of the individual linear regressions from log (% ISF Aβ40) versus time. Data are mean ± SEM. *p < 0.05 (two-tailed Student's t test). C, D, Full-length APP (C) and sAPPα and sAPPβ levels (D) in the hippocampus and cortex of APP/PS1 and APP/PS1; aLrp1−/− mice (n = 8–10/group) at 12 months of age were analyzed by Western blotting and quantified. Data are mean ± SEM. E, AQP-4 in the cortex of APP/PS1 and APP/PS1; aLrp1−/− mice (n = 8–10/group) at 12 months of age were analyzed by Western blotting and quantified. Data are mean ± SEM.