Figure 3.
Requirement of the TIM23-translocase for prohibitin biogenesis. (A) Depletion of Tim23 in vivo. Mitochondial membranes were prepared from Tim23(Gal10) cells that were shifted for 24 h to galactose-free medium (Tim23↓). Samples were analyzed by SDS-PAGE and immunodecoration. YPH499 cells were analyzed equally for a control (WT). (B) Import of prohibitins into Tim23-deficient mitochondria. 35S-labeled precursor proteins of Phb1, Phb2, Su9(1-69)-DHFR, and Tim23 were imported into mitochondria isolated from wild-type (mb2) (□) or Tim23(fs) (▴) cells. Protease-resistant, imported protein was quantified by phosphorimaging.