Figure 6.

Modulation of signal-mediated sequestration in cis. Sets of constructs containing the indicated signal sequences, with or without 10 amino acids from the respective mature domains (gray and black bars, respectively), were analyzed for luciferase activity and total expression. Except for the Lep and PrP signals, a frameshift mutation (Figure 4A) was analyzed in parallel (white bars) to assess the contribution of leaky ribosomal scanning to the luciferase activity observed for the respective Signal₁₀-TF constructs. Ten nanograms of plasmid per well was analyzed in each instance. Due to the large differences in absolute luciferase activity among different signals (Figure 5B), each set of constructs was plotted separately and normalized to the respective SS-TF construct (set to 100%). Note that the 10 amino acids from the mature domain can increase, decrease, or have little effect on the efficiency of TF sequestration.