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cAMP-binding energies and cooperativities of CRPSC variants. Light and dark squares correspond to the cAMP-binding energy for the first (ΔG01) and second (ΔG02) sites, respectively. The order of CRPSC mutants is sorted from most positive cooperativity (ΔG02 − ΔG01 < 0) to most negative (ΔG02 − ΔG01 > 0) as indicated by the dashed lines.
