Table 1.
cAMP and DNA binding affinity constants to CRPSC
Error corresponds to standard deviation from fitted parameters using a two-site binding model as described in Equation 1 under “Materials and Methods.” The units of k1 and k2 are 104 m−1 and c = k2/k1. The units of kDNA(empty) and kDNA(cAMP-2) are 107 m−1.
| cAMP binding affinity and cooperativity (c) |
DNA binding affinity |
||||
|---|---|---|---|---|---|
| k1 | k2 | c | kDNA(empty) | kDNA(cAMP-2) | |
| CRPSCWT/WT | 3.4 ± 0.6 | 6.1 ± 2.3 | 1.82 | 0.6 ± 0.1 | 6.8 ± 0.7 |
| CRPSCS/S | 0.34 ± 0.06 | 0.04 ± 0.03 | 0.12 | 1.2 ± 0.1 | 6.3 ± 0.1 |
| CRPSCD/D | 4.4 ± 1.9 | 38.9 ± 8.9 | 8.79 | 1.0 ± 0.2 | 20.2 ± 1.7 |
| CRPSCS/WT | 2.3 ± 0.3 | 0.47 ± 0.06 | 0.20 | 0.5 ± 0.1 | 23.0 ± 4.0 |
| CRPSCD/WT | 4.2 ± 0.8 | 17.1 ± 3.4 | 4.08 | 0.3 ± 0.1 | 19.8 ± 1.0 |
| CRPSCS/D | 2.8 ± 0.7 | 0.7 ± 0.3 | 0.25 | 0.7 ± 0.1 | 8.9 ± 2.3 |
| CRPSCS+D/WT | 1.5 ± 0.2 | 1.7 ± 0.2 | 1.12 | 1.1 ± 0.2 | 20.0 ± 7.4 |