BLT1 and TRPV1 mediate LTB4-induced thermal hyperalgesia.
A, thermal withdrawal thresholds after injection of 10 μl of LTB4 (20 ng) or vehicle in one hind paw of wild-type mice. The data are shown as means ± S.E. (n = 6; two-way ANOVA; Bonferroni post correction). *, p < 0.05; **, p < 0.01. B, FACS determination of F4–80 (macrophages)- and Ly6G (neutrophils)-positive cells in the paw of untreated wild-type mice and 1 or 2 h after LTB4 injection. The data are shown as means ± S.E. (n = 4–6; one-way ANOVA; Bonferroni post hoc test). *, p < 0.05. C, thermal paw withdrawal latencies before and after injection of 10 μl of LTB4 (20 ng) in one hind paw of wild-type or BLT1 knock-out mice. The data are show as means ± S.E. (n = 7–8; two-way ANOVA; Bonferroni post correction). **, p < 0.01. D, thermal paw withdrawal latencies before and after injection of 10 μl LTB4 (20 ng) in one hind paw of wild-type or TRPV1 knock-out mice. The data are shown as means ± S.E. (n = 6; two-way ANOVA; Bonferroni post correction). ***, p < 0.001. PWL, paw withdrawal latency.