Table 1.
Oligonucleotides for cloning gRNAs
| Oligo name | Sense oligo sequence |
|---|---|
| Apc_t1_LA | CACCGTAAAGTATTTGTCTGGCTC |
| Apc_t1_RA | CACCGCATTATTGAAAGTGGAGGT |
| Apc_t2_LA | CACCGATACCGTAGTATTTAAATA |
| Apc_t2_RA | CACCGCCCAGCTCTTCTTCCTCAA |
| Apc_t3_LA | CACCGCGCAGCTGATTCTGCCTTT |
| Apc_t3_RA | CACCGCAAGTTCAGCACAAAGAAA |
| Pten_t1_LA | CACCGCTAACGATCTCTTTGATGA |
| Pten_t1_RA | CACCGAAAAGGAGATATCAAGAGGA |
| Pten_t2_LA | CACCGTGATAAGTTCTAGCTGTGGT |
| Pten_t2_RA | CACCGTCTGTGAAGATCTTGACCAA |
| Pten_t3_LA | CACCGTGTCATCTTCACTTAGCCAT |
| Pten_t3_RA | CACCGCAGCAATTCACTGTAAAGC |
| Smad4_t1_LA | CACCGACTATGTACAATGCTCAGAC |
| Smad4_t1_RA | CACCGTTGATGTGTCATAGACAAGG |
| Smad4_t2_LA | CACCGCGCGATTACTTGGCGGGTGT |
| Smad4_t2_RA | CACCGAACGGAGACGTACAGCGCCC |
| Smad4_t3_LA | CACCGATGGCTGCCCGCCAGAATAC |
| Smad4_t3_RA | CACCGGACTGTTGCAGATAGCTTC |
| P53_t1_LA | CACCGCAGGAGCTCCTGACACTCGG |
| P53_t1_RA | CACCGACCCTGTCACCGAGACCCC |
| P53_t2_LA | CACCGTTCCACCCGGATAAGATGCT |
| P53_t2_RA | CACCGAAATTTGTATCCCGAGTATC |
| P53_t3_LA | CACCGTGCCTGTCTTCCAGATACTC |
| P53_t3_RA | CACCGACTTTTCGCCACAGCGTGG |
| NFE2L2_T1_PSL | CACCGGGATCTGCCAACTACTCCC |
| NFE2L2_T1_PSR | CACCGGGTTTCTGACTGGATGTGC |
| mLet7g_T2_PSL | CACCGAGCGCTGGAGCAGCTCTGC |
| mLet7g_T2_PSR | CACCGTTCTCTTTTGCCTGATTCC |
| AKT_PSR | CACCGAGGGTTGGCTGCACAAACG |
| AKT_PSL | ACACCGACAATAGCCACGTCGCTCAG |
| Non-specific (NS) | CACCGGGTCTTCGAGAAGACCT |