Table 2.

Oligos for generating paired nickase target sites in target transposon vectors

The sense strand of the right target site is shown in bold. The antisense strand of the left target site is underlined.

Target	Target sequence (cloned into BstEII site of BII-CPspaH2BG/CPspaH/C3H/B3HTK)
Apc_t1	CCGGAGCCAGACAAATACTTTAGCCATTATTGAAAGTGGAGGTGGG
Apc_t2	CCATATTTAAATACTACGGTATTGCCCAGCTCTTCTTCCTCAAGGG
Apc_t3	CCAAAAGGCAGAATCAGCTGCGTCCAAGTTCAGCACAAAGAAATGG
Pten_t1	CCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGG
Pten_t2	CCCACCACAGCTAGAACTTATCAAACCCTTCTGTGAAGATCTTGACCAATGG
Pten_t3	CCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTAAAGCTGG
Smad4_t1	CCTGTCTGAGCATTGTACATAGTTTGATGTGTCATAGACAAGGTGG
Smad4_t2	CCAACACCCGCCAAGTAATCGCGCATCAACGGAGACGTACAGCGCCCCGG
Smad4_t3	CCAGTATTCTGGCGGGCAGCCATAGTGAAGGACTGTTGCAGATAGCTTCAGG
P53_t1	CCTCCGAGTGTCAGGAGCTCCTGCAGCACAGGACCCTGTCACCGAGACCCCTGG
P53_t2	CCCAGCATCTTATCCGGGTGGAAGGAAATTTGTATCCCGAGTATCTGG
P53_t3	CCCGAGTATCTGGAAGACAGGCAGACTTTTCGCCACAGCGTGGTGG
NFE2L2_T1	CCAGCACATCCAGTCAGAAACCAGTGGATCTGCCAACTACTCCCAGG
mLet7g_T2	CCTGCAGAGCTGCTCCAGCGCTCCGTTCTCTTTTGCCTGATTCCAGG
AKT	CCATGAGCGACGTGGCTATTGTGAAGGAGGGTTGGCTGCACAAACGAGG