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. 2017 Feb 22;292(15):6148–6162. doi: 10.1074/jbc.M117.777722

Table 4.

Oligos and primers used for targeting AKT

*, phosphorothioate linkage.

Primer/oligo name Sequence Notes
myrAKT ssODN G*A*GAGGAGCGCGTGAGCGTCGCGGGAGCCTCGGGCACCATGGGGAGCAGCAAGAGCAAGCCCAAGTCTAGAATGAACGACGTGGCTATTGTGAAGGAGGGTTGGCTGCACAAACGAGGTTAGTA*C*C Donor oligo for knock-in
PAGE-purified
MTOR_S2215Y ssODN C*T*TCGGCCTGGTTAACACCCTTCTGGCCAATGACCCAACATATCTTCGGAAAAACCTCAGGTATTCAGAAACCCTGAAATGGCA*A*C Negative control (non-specific) donor oligo
PAGE-purified
AKT_990 (F) CTTCCTTTGCTTCTCCCAGAG QPCR (SYBR or Taqman) with AKT_1146(R)
AKT_1146 (R) CTCGTTTGTGCAGCCAAC QPCR (SYBR or Taqman) with AKT_990(R)
AKT_probe GAGCAGCAAGAGCAAGCCCAAG Taqman probe for QPCR with AKT_990(F) and AKT_1146(R)