Figure 4.

Activated CDK5 induces degradation of EZH2. A, PANC-1 cells were transfected with the indicated plasmids for 24 h, followed by Western blotting analysis. Data are representative of multiple experiments (n = 3). IB, immunoblot. B, Western blotting analysis of WCLs of PANC-1 cells transfected with the indicated constructs. Cells were treated with 20 μm MG132 for 8 h before harvest or left untreated. Data are representative of multiple experiments (n = 3). C and D, PANC-1 cells were transfected with the indicated plasmids for 24 h, followed by Western blotting analysis. Data are representative of multiple experiments (n = 3). E and F, PANC-1 cells were transfected with the indicated plasmids. After 24 h, cells were treated with 50 μg/μl CHX. At different time points, cells were harvested for Western blotting analysis. G, PANC-1 cells were transfected with the indicated plasmids for 16 h, followed by treatment with 20 μm MG132 for 8 h. Immunoprecipitated FLAG-EZH2 proteins were analyzed by WB for ubiquitination.