Figure 6.

FBW7 inhibits tumor migration and invasion via degradation of EZH2 in pancreatic cancer cells. A, E, and I, PANC-1 cells and MIA PaCa-2 cells were transfected with the indicated constructs and cultured to confluence on 6-well plates. The cell layer was scratched with a 200-μl pipette tip. For each sample, at least three scratched fields were photographed immediately (0 h) or 48 h after scratching. Photographs of representative images were taken at ×100 magnification. C, G, and K, quantification of cell migration by ImageJ after scratching of PANC-1 and MIA PaCa-2 cells. *, p < 0.05; n.s., not significant. B, D, F, H, J, and L, PANC-1 and MIA PaCa-2 cells were transfected with the indicated constructs. 48 h after transfection, cells were used for Matrigel invasion assays. Representative images of invasion assay are shown in B, F, and J, and the quantification results are shown in D, H, and L, respectively. Scale bars = 200 μm. Data are mean ± S.D. from experiments with three replicates. *, p < 0.05.