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Representative ITC thermograms obtained by the successive addition of Itch PRR to a subset of SH3 domains. A, representative results obtained by ITC for the binding of the 249–269 peptide to the SH3 domain of endophilin, pacsin, β-PIX, and amphiphysin. B, the calculated dissociation constant (KD) and stoichiometries (N) are compiled with results obtained previously using a longer 224–276 peptide (8).
