Figure 2.

TG-dependent incorporation of DCA into rRelish-N without the GST tag. A, rRelish-N digested with PreScission Protease (2 units) was incubated with rTG in the presence of DCA (1 mm) for different incubation times. Treated proteins were separated by SDS-PAGE and detected by UV illumination (left) and CBB staining (right). B, CBB-stained bands and the DCA fluorescence were quantitated using ImageJ software. Data for A and B are representative of three independent experiments.