Figure 3.

Polymerization of rRelish-N by TG. A, rRelish-N was incubated with rTG. Treated proteins were separated by SDS-PAGE and detected by Western blotting. B, rRelish-N (5 nm) was incubated with rTG in the presence of rRelish-C (5 nm). Treated proteins were separated by SDS-PAGE and detected by Western blotting. C, the intensity of rRelish-N (monomer) was analyzed by UltraQuant ID Gel Analysis Software (Aplegen). Error bars represent standard errors of mean values (n = 3). *, p < 0.05. D, rRelish-N (5 nm) was incubated with rTG in the presence of different concentrations of rRelish-C (0, 5, 50, and 500 nm). The intensity of the rRelish-N (monomer) band was analyzed by ImageJ software. E, dimethylcasein (5 nm) was incubated with rTG in the presence of different concentrations of rRelish-C (0, 5, 50, and 500 nm). The intensity of the DCA-incorporated dimethylcasein band was analyzed by ImageJ software. Data for A, B, D, and E are representative of three independent experiments.