Table 1.

Oligonucleotide primers used in this study

Sequences are given in 5′ to 3′ orientation; F (forward) and R (reverse) refer to the direction of priming. In some cases, additional bases were added at the 5′-end for increased efficiency of restriction. Restriction sites are underlined, and the restriction enzyme used is indicated in the primer name. All oligonucleotides were obtained from Eurofins Genomics GmbH (Ebersberg, Germany). The His₆ tag (His), FLAG tag (FLAG) and deletions (Δ) are indicated in the primer name and the name of the product. In the case of primers binding inside the cDNA sequence, position is counted from the start codon.

Primer sequence	Name	Product
CCATGGCTAAGCTCTCTCTTTCC	SBT1.4-F	SBT1.4
CCTCAGAAGGACTGAACTGATCCCTG	SBT1.4-R	SBT1.4
TCATGTCTTCTTCGTTTCTCTCCT	SBT1.7-F	SBT1.7
GACTATGTCCAGCTAATCGCCAC	SBT1.7-R	SBT1.7
ATGGCTTCTTCTTCTTCTTCTTCTT	SBT1.8-F	SBT1.8
TATCAAAACCGGTTCCAAGAGAAT	SBT1.8-R	SBT1.8
GGCTCGAGATGGCGAATCTAGCTGCGTC	SBT4.12-XhoI-F	SBT4.12-His
GGATCCTCAGTGATGGTGATGGTGATGTGCCTCATCAACAACCATTATG	SBT4.12-BamHI-His-R	SBT4.12-His
CGCTCGAGATGGCGACGCTAGCAG	SBT4.13-XhoI-F	SBT4.13-(His/PP)
CGCCCGGGTCAGTAATCACTAGTATAAACAACAATGGG	SBT4.13-SmaI-R	SBT4.13
GGATCCTCAGTGATGGTGATGGTGATGGTAATCACTAGTATAAACAACA	SBT4.13-BamHI-His-R	SBT4.13-His
AAGTCCCAAGACGTCGTCGTAGCTGAAACTGAACTCAAGAACAAA	SBT4.13-SP-72 R	SBT4.13ΔPP
TTTGTTCTTGAGTTCAGTTTCAGCTACGACGACGTCTTGGGACTT	SBT4.13-327-F	SBT4.13ΔPP
CCGGCGGAATTCACAGTGAGAA	SBT4.13-894-R	SBT4.13ΔPP
GGCTCGAGATGAAAGGCATTACATTCTTCACA	SBT5.2-XhoI-F	SBT5.2-His
GGATCCTCAGTGATGGTGATGGTGATGGTTTGTGCGGCTACTCTCGCTAC	SBT5.2-His-BamHI-R	SBT5.2-His
CGCTCGAGATGCAAACATTTGCTCCTGGAAC	SPI-XhoI-F	SPI-FLAG
GGCCCGGGTCATTTGTCGTCATCATCCTTGTAGTCGGTCAACTTGAAACCACCACCA	SPI-FLAG-SmaI-R	SPI-FLAG
GTCCCGGGTCATTTGTCGTCATCATCCTTGTAGTCTTGAAGTTGTAACTTCTTGTTTGGG	SBT4.13PP-FLAG-SmaI-R	SBT4.13PP
CCCATATGCACCACCACCACCACCACGATGAATATACCGGAGAAGCAACGG	SPI-His-NdeI-104-F	SPI-His
CCGTCGACTTAGGTCAACTTGAAACCACCACC	SPI-SalI-R	SPI-His