Figure 7.

Knockdown of CARM1 protein promotes post-synaptic clustering of NR2B and PSD-95 proteins. A–C, cultured hippocampal neurons were untreated or transduced with control (Cont) or CARM1-specific siRNA at 9 DIV and incubated for 5 days. At 14 DIV, neurons were fixed and used for double-staining with CARM1 and synapsin (A), NR2B (B), or PSD-95 (C) antibodies. Scale bar, 10 μm. D and E, Synaptic cluster size (μm²) and synaptic clustering (number per 10 μm) were calculated and statistically analyzed. Mean ± S.E., three independent experiments. *, p < 0.05, **, p < 0.01, compared with untreated).