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Quantitative PCR experiments to show the cell-density-dependent effect of NGF on Hes1/5 mRNA levels. Hippocampal neurons were for 7 DIV cultured at low (LD, 15,000 cells/cm2) or high (HD, 150,000 cells/cm2) cell density and for 3.5 h incubated in the absence or presence of NGF (100 ng/ml). Observe that NGF increased Hes1/5 mRNA levels only in low cell density cultures.
