Figure 2.

Hypersensitivity of lsk1Δ mutants to LatA stems from an inability to complete septum formation. (A) Cells of the indicated genotype were grown to mid-log phase at 32°C and then treated with 0.3 μM LatA for 5 h before being fixed and stained with DAPI (nuclei) and aniline blue (cell wall/septa). Bar, 10 μm. (B) Wild-type and lsk1Δ mutants were grown to mid-log phase at 32°C and treated for 20 min with the indicated concentration of LatA. Cells were subsequently fixed and stained with Alexafluor-488 phalloidin (actin) and DAPI (nuclei). Bar, 10 μm.