Figure 7.

Loss of Lsk1p does not prevent activation of the cytokinesis checkpoint. (A) Wild-type and lsk1Δ cells carrying an integrated GFP-tagged version of Clp1p were synchronized in early G2 phase by centrifugal elutriation and then released into YES media containing 0.3 μM LatA or DMSO at 32°C. Cells were subsequently fixed and examined using GFP autofluorescence. Bar, 10 μm. (B) Wild-type and lsk1Δ cells carrying an integrated GFP-tagged version of Cdc7p were grown to mid-log phase and treated with 0.3 μM LatA or DMSO for 3 h. Cells were subsequently, fixed and stained with DAPI (nuclei) and antibodies specific for microtubules and GFP (see Materials and Methods). Bar, 5 μm.