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OSCC cells were treated with BAY11-7082 1 h before TNF-α stimulation for the indicated times. (A) The mRNA level of IKKβ and p65 was analyzed by real-time RT-PCR. β-actin was used as a control. (B) Western blot analysis was performed to assess the expression of IKKβ and p65 on the protein level. β-actin was employed as a loading control. (C) The statistics analysis of western blot analysis. Each bar represents the mean ±S.D. The difference was not significant, P>0.05.
