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. 2017 Apr 10;12:2899–2911. doi: 10.2147/IJN.S131418

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© 2017 Mu et al. This work is published and licensed by Dove Medical Press Limited

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Confirmation of the bioconjugation of EGFR antibody to Fe₃O₄@PDA NPs.

Notes: (A) TEM image and (B) size distribution histogram of the Fe₃O₄@PDA NPs. (C) FT-IR spectra of Fe₃O₄@PDA and Fe₃O₄@PDA-PEG. (D) SDS-PAGE analysis of Fe₃O₄@ PDA-PEG-EGFR and EGFR antibody. The Coomassie blue-stained gel analysis revealed the successful cross-linking of EGFR antibody molecules on the surface of the Fe₃O₄@PDA-PEG NPs. Lane 1, EGFR antibody; lanes 2 and 3, the bioconjugated Fe₃O₄@PDA-PEG-EGFR NPs; lane 4, protein molecular weight marker; lane 5, Fe₃O₄@PDA-PEG NPs.

Abbreviations: FT-IR, Fourier transform–infrared; SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; TEM, transmission electron microscope; PDA, polydopamine; PEG, polyethylene glycol; NP, nanoparticle.