(A) Total mRNA levels of E-selectin, MCP-1, VCAM-1, and ICAM-1 in thoracic aortas from cortactin+/+ and cortactin+/− mice, data are mean ± SEM from 5 mice in each group. (B) Intima was further dissected from the aortic specimens and the remaining tissue was defined as media and adventitia, and the mRNA levels of E-selectin, MCP-1, VCAM-1, and ICAM-1 were then quantified. Data are mean ± SEM from 6 mice in each group. (C) ApoE−/−/cotactin+/+ mice (n = 15) and ApoE−/−/cotactin+/− (n = 11) littermates were fed on atherogenic diet for 10 weeks. Atherosclerosis in mouse aortas was stained with Oil-red O. The macrophotographs shown are representative of results from the two groups. (D, E, F) Scatterplots and quantification of percentage lesion areas (with perpendicular lines indicating mean) in the thoracic aorta, aortic arch, and a sum of the whole aorta. ‘*’ indicates P < 0.05.