Figure 3.

LPL and EL mRNA expression in differentiated 3T3-L1 cells and isolated mouse adipocytes. Fat pads of mice in the presence (L2) or absence (L0-MCK) of LPL were homogenized and subjected to collagenase A digestion, and total RNA of adipocytes was isolated. Total RNA from 3T3-L1 fibroblasts was isolated at 50% cell confluence (day –2), at confluence (day 0) and during (days 2, 4, 6, and 7), and at the end of differentiation (day 8) to adipocytes. (A) Northern blot analysis of 10 μg total RNA per lane, hybridized with a murine-specific [³²P]-labeled LPL probe. (B) EL mRNA concentrations were determined by fluorescent real-time PCR. EL mRNA quantities were normalized to those of β-actin, and control values from L2 mice were arbitrarily set to 1. *P < 0.05.