Table S1.

Lyme disease serology test performance based on clinical samples

Stage	Test method	Sensitivity	Specificity
Single test	C6 ELISA	33.1%	92.1%
First tier with equivocals	C6 ELISA	36.9%	92.1%
Single test	ELISA	38.3%	96.8%
Single- or two-tier test	Western blot	38.3%	94.8%
Combined	Two tier	33.0%	99.7%
Average	All	36.5%	95.8%

Notes: When C6 ELISA is used as a single test, only positives are reported. When used as the first stage of a two-tier test, both positives and equivocals are included for the confirmatory Western blot.

Abbreviation: ELISA, enzyme-linked immunosorbent assay.