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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Surg Res. 2016 Apr 25;204(1):145–152. doi: 10.1016/j.jss.2016.04.034

Figure 1. TNF-α stimulates myofibroblast migration via p38 MAPK.

Figure 1

The surface of a confluent monolayer of 18Co cells was scratched with a sterile pipette tip and incubated in serum free medium. Three pictures were randomly taken along the scratch gap and pictured positions were marked (time 0 h). Cells were then treated with 10 ng/ml TNF-α for 24 h, in the presence or absence of SB203580 (5 μM), an inhibitor of p38 MAPK. Three pictures were taken after 24 h at the same positions as marked previously. The scratch gap area was measured with software Image J and normalized to time 0 control. The results shown are the mean ± S.E. n ≥ 3 and are expressed as percentage change in scratch gap area. * denotes p < 0.05.