Table 1. Relative abundance of distinct metabolites in Tg and WT astrocytes, treated or not with pantethine.
Intracellular levels of different metabolites were determined. The table shows normalized data expressed relative to the abundance of the untreated WT group and are shown as the mean values ± SD from three independent experiments (n = 3 per group); (*, values significantly different between Tg and WT astrocytes; #, values significantly different compared to the corresponding untreated groups; p≤0.05).
| Cell type | WT | WT | Tg | Tg |
|---|---|---|---|---|
| Pantethine | − | + | − | − |
| Glycolytic pathway | ||||
| PEP | 1±0.2 | 1.07±0.19 | 1.39±0.15* | 1.43±0.13 |
| G6-P | 1±0.19 | 0.95±0.17 | 0.63±0.08* | 0.54±0.05 |
| Sed7P | 1±0.15 | 0.91±0.19 | 0.47±0.09* | 0.64±0.08# |
| Gly-3P | 1±0.19 | 0.97±0.16 | 0.37±0.1* | 0.53±0.08# |
| TCA cycle metabolites | ||||
| α-KG | 1±0.12 | 0.94±0.13 | 1.25±0.15* | 0.56±0.11# |
| Succinate | 1±0.13 | 0.99±0.12 | 0.92±0.11 | 0.72±0.09# |
| Fumarate | 1±0.21 | 0.94±0.19 | 0.65±0.09* | 0.99±0.13# |
| Cis-aconitate | 1±0.25 | 0.94±0.18 | 1.37±0.3 | 1.29±0.28 |
| Citrate | 1±0.25 | 0.86±0.21 | 1.24±0.29 | 1.26±0.22 |
| Nucleotides | ||||
| ATP | 1±0.08 | 0.97±0.12 | 1.01±0.13 | 1.44±0.14# |
| AMP | 1±0.07 | 0.90±0.19 | 0.80±0.11* | 1.07±0.15 |
| CMP | 1±0.15 | 0.96±0.19 | 0.60±0.11* | 1.00±0.22# |
| CTP | 1±0.12 | 0.98±0.15 | 1.03±0.11 | 1.16±0.13 |
| GDP | 1±0.15 | 0.95±0.13 | 0.91±0.11 | 1.03±0.16 |
| ADP | 1±0.18 | 0.98±0.16 | 1.10±0.12 | 1.19±0.16 |