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Electrostatic potential of the following residues: (A) native R131, (B) R131Q (C) R131W, (D) native R203, and (E) R203C. R131 in the native protein (A) was pale red in color and retained same color even after substituted with Tryptophan (B) and Glutamine (C). However, R203 in the native protein (D) was deeper red in color (larger positive charge), which subsequently changed to blue color (E) after substituted with Cysteine.
