Figure 1.

Generation of β cell–specific PKCλ knockout mice. (A) PCR analysis of genomic DNA isolated from islets of control (PKCλ^flox/flox) and βPKCλ^–/– mice. The primers were targeted to regions external to the loxP sites of PKCλ^flox/flox mice. (B and C) Immunoblot analysis of PKCλ in the islets (B) or the brain, hypothalamus, heart, liver, skeletal muscle, and fat (C) of control and βPKCλ^–/– mice. Tissue homogenates were subjected to immunoprecipitation and subsequent immunoblot analysis with antibodies against PKCλ. The expression of PKCλ in islets was similarly analyzed. (D) Immunostaining of islets in pancreatic sections of control and βPKCλ^–/– mice with antibodies against PKCλ and FITC-conjugated secondary antibodies. Scale bars: 50 μm.