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. 2017 Apr 14;12(4):e0175655. doi: 10.1371/journal.pone.0175655

Table 5. Potential N-linked glycosylation sites of NA proteins in seasonal influenza strains circulating in Thailand.

NA position NA sequences N-Gly Scorea Jury agreementb N-Gly resultsc Clade
A(H1N1) seasonal (N = 118)
44 NHTG 0.671 (8/9) + All
58 NSTW 0.599 (8/9) + All
63 NHTY 0.560 (8/9) + All
70 NNTN 0.688 (8/9) + All
88 NSSL 0.767 (9/9) +++ All
146 NGTV 0.687 (9/9) ++ All
235 NGSC 0.736 (9/9) ++ All
434 NTTI 0.610 (7/9) + All
455 NWSW 0.272 (9/9) --- All
A(H1N1)pdm09 (N = 296)
42 NQSQ 0.629 (8/9) + Only clade 6B, 6C
50 NQSV 0.554 (6/9) + All
58 NNTW 0.569 (6/9) + All
63 NQTY 0.681 (9/9) ++ All
68 NISN 0.737 (9/9) ++ All
88 NSSL 0.738 (9/9) ++ All
146 NGTI 0.660 (9/9) ++ All
235 NGSC 0.728 (9/9) ++ All
386 NFSI 0.294 (9/9) --- All except clade 6A and 6B
A(H3N2) (N = 343)
61 NITE 0.756 (9/9) +++ All
70 NTTI 0.545 (6/9) + All
86 NWSK 0.604 (8/9) + All
93 NITG 0.685 (9/9) ++ Only clade WI/05
146 NDTV 0.642 (9/9) ++ All
200 NATA 0.361 (9/9) -- All
234 NGTC 0.755 (9/9) +++ All
329 NDSS 0.488 (5/9) - All except Br/07
367 NETS 0.540 (5/9) + 3A, 3B, 3C.2, 3C.3, 4 and 6
402 NRSG 0.407 (6/9) - All except 3B, 3C and 4
Influenza B virus (N = 232)
56 NASN 0.592 (7/9) + All
64 NRSA 0.685 (8/9) + All
144 NGTR 0.816 (9/9) +++ All
284 NKTI 0.704 (9/9) ++ All
463 NMTL 0.425 (6/9) - Only clade Yam-3

a The potential score is the averaged output of nine neural networks

b The Jury agreement indicate how many of the nine networks support the prediction

c The N-Glyc result indicates (+) potential N-glycosylated sites > 0.5 threshold, (++) potential N-glycosylated sites > 0.5 threshold and Jury agreement (9/9), (+++) potential N-glycosylated sites > 0.75 threshold and Jury agreement (9/9), (-) non-glycosylated sites < 0.5 threshold, (- -) non-glycosylated sites < 0.5 threshold and Jury agreement (9/9), and (- - -) non-glycosylated sites < 0.32 threshold.