Table 5. Potential N-linked glycosylation sites of NA proteins in seasonal influenza strains circulating in Thailand.
| NA position | NA sequences | N-Gly Scorea | Jury agreementb | N-Gly resultsc | Clade |
|---|---|---|---|---|---|
| A(H1N1) seasonal (N = 118) | |||||
| 44 | NHTG | 0.671 | (8/9) | + | All |
| 58 | NSTW | 0.599 | (8/9) | + | All |
| 63 | NHTY | 0.560 | (8/9) | + | All |
| 70 | NNTN | 0.688 | (8/9) | + | All |
| 88 | NSSL | 0.767 | (9/9) | +++ | All |
| 146 | NGTV | 0.687 | (9/9) | ++ | All |
| 235 | NGSC | 0.736 | (9/9) | ++ | All |
| 434 | NTTI | 0.610 | (7/9) | + | All |
| 455 | NWSW | 0.272 | (9/9) | --- | All |
| A(H1N1)pdm09 (N = 296) | |||||
| 42 | NQSQ | 0.629 | (8/9) | + | Only clade 6B, 6C |
| 50 | NQSV | 0.554 | (6/9) | + | All |
| 58 | NNTW | 0.569 | (6/9) | + | All |
| 63 | NQTY | 0.681 | (9/9) | ++ | All |
| 68 | NISN | 0.737 | (9/9) | ++ | All |
| 88 | NSSL | 0.738 | (9/9) | ++ | All |
| 146 | NGTI | 0.660 | (9/9) | ++ | All |
| 235 | NGSC | 0.728 | (9/9) | ++ | All |
| 386 | NFSI | 0.294 | (9/9) | --- | All except clade 6A and 6B |
| A(H3N2) (N = 343) | |||||
| 61 | NITE | 0.756 | (9/9) | +++ | All |
| 70 | NTTI | 0.545 | (6/9) | + | All |
| 86 | NWSK | 0.604 | (8/9) | + | All |
| 93 | NITG | 0.685 | (9/9) | ++ | Only clade WI/05 |
| 146 | NDTV | 0.642 | (9/9) | ++ | All |
| 200 | NATA | 0.361 | (9/9) | -- | All |
| 234 | NGTC | 0.755 | (9/9) | +++ | All |
| 329 | NDSS | 0.488 | (5/9) | - | All except Br/07 |
| 367 | NETS | 0.540 | (5/9) | + | 3A, 3B, 3C.2, 3C.3, 4 and 6 |
| 402 | NRSG | 0.407 | (6/9) | - | All except 3B, 3C and 4 |
| Influenza B virus (N = 232) | |||||
| 56 | NASN | 0.592 | (7/9) | + | All |
| 64 | NRSA | 0.685 | (8/9) | + | All |
| 144 | NGTR | 0.816 | (9/9) | +++ | All |
| 284 | NKTI | 0.704 | (9/9) | ++ | All |
| 463 | NMTL | 0.425 | (6/9) | - | Only clade Yam-3 |
a The potential score is the averaged output of nine neural networks
b The Jury agreement indicate how many of the nine networks support the prediction
c The N-Glyc result indicates (+) potential N-glycosylated sites > 0.5 threshold, (++) potential N-glycosylated sites > 0.5 threshold and Jury agreement (9/9), (+++) potential N-glycosylated sites > 0.75 threshold and Jury agreement (9/9), (-) non-glycosylated sites < 0.5 threshold, (- -) non-glycosylated sites < 0.5 threshold and Jury agreement (9/9), and (- - -) non-glycosylated sites < 0.32 threshold.