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. 2017 Apr 14;12(4):e0175712. doi: 10.1371/journal.pone.0175712

Fig 3. Levels of various immunomodulatory factors in OC-associated ascites and their correlation with the immunosuppressive effects observed in DC activation assays.

Fig 3

(A) Protein levels of various factors in ascites samples collected from OC patients were measured by sandwich ELISA. Eight malignant ascites samples used throughout the study are shown. Each symbol represents one patient sample throughout the graphs (n = 8). (B) Levels of IL-10 in ascites samples are correlated to the suppression of TLR-mediated up-regulation of CD86 and production of IL-6, IL-12 and TNFα. Suppression is expressed in per cent reduction of surface marker and cytokine levels when 10% ascites was added to the cell culture as compared to no ascites present; n = 26 (26 independent experiments conducted with monocyte-derived DC from 9 different healthy controls, cultured with ascites from two (n = 4), three (n = 2) or four (n = 3) different OC patients. The Pearson coefficient was calculated to assess statistical significance of correlations.