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. 2017 Apr 14;12(4):e0175712. doi: 10.1371/journal.pone.0175712

Fig 6. The suppression of DC activation by peritoneal fluid from patients with beningn conditions is fully mediated by IL-10 unlike the suppressive activity of OC-associated ascites.

Fig 6

Monocyte-derived DC were stimulated overnight with 3μg/ml R848 in the presence or absence of (A) 10% malignant ascites from OC patients or (B) 10% peritoneal fluid from benign ovarian tumours and IL-10 neutralizing antibody (5μg/ml) as indicated. CD86 expression levels were assessed by flow cytometry, and cytokine levels were measured in cell culture supernatants by flow cytomix analysis (A: IL-6 and TNFα) or sandwich ELISA (A: IL-12p40 and B: TNFα, IL-6 and IL-12p40); for malignant samples n = 6 (6 independent experiments; monocyte-derived DC from 5 different healthy donors, cultured with 1 (n = 4), or 2 (n = 1) different ascites samples); for benign samples n = 9 (9 independent experiments; monocyte-derived DC from 5 different healthy donors, cultured with 1 (n = 3), or 3 (n = 2) different peritoneal fluids); One-way ANOVA (Friedman test with Dunn post test): * = p<0.05, ns = not significant.