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. 2017 Mar 30;12:1177271917699850. doi: 10.1177/1177271917699850

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — PCR efficiency and linearity for HRG and 3 housekeeping genes: (A) HRG^a,b (B) HMBS^b, (C) EIF2B1^b, and (D) IPO8^b. Ct values as a function of input template. Ct indicates cycle threshold.

^aThe HRG figure represents a 7-point curve analysis due to no amplification seen in lowest point of 0.01 ng RNA.

^bEach point represents the mean Ct across 6 runs.