Table 3.
HRG gene expression assay analysis in frozen NSCLC samples.a
| RNA origin |
Ct values for HRG primer/probe Cb |
|
|---|---|---|
| Mean | SD | |
| Frozen NSCLC | ||
| 1173692F-30 | 28.2 | 0.05 |
| 1173127F-30 | 29.8 | 0.05 |
| 1173740F-30 | 32.0 | 0.19 |
| 2YHOD | 28.6 | 0.02 |
| AF8AL | 27.8 | 0.06 |
| Q151Z | 25.4 | 0.02 |
| TRQR7 | 25.6 | 0.09 |
| Mean | 28.2 | |
| SD | 2.29 | |
| Fresh cell linesc | ||
| HRG+ | 25.5 | 0.05 |
| HRG(−) | 38.8 | NC |
Abbreviations: Ct, cycle threshold; HRG, heregulin; NC, could not be calculated; NSCLC, non–small cell lung cancer.
a
Three HRG gene expression assays (only primer/probe C is shown) were tested in triplicate on 7 frozen NSCLC samples and 2 cell line RNA controls. Cell line RNA controls were included to correlate with previous work and not used in the final calculations for the mean and SD.
b
Primer/probe C = Hs01101537_m1.
c
HRG-positive cell line = A549 and HRG-negative cell line = T47D.