Table 5.

Comparison of Ct values in matched frozen to FFPE NSCLC tissue with HRG plus 5 reference genes.

	Mean Ct value
	HRG (72 bp)			HMBS (64 bp)			EIF2B1 (75 bp)
	Frozen NSCLC	FFPE NSCLC	ΔCt FFPE vs frozen NSCLC	Frozen NSCLC	FFPE NSCLC	ΔCt FFPE vs frozen NSCLC	Frozen NSCLC	FFPE NSCLC	ΔCt FFPE vs frozen NSCLC
FFPE NSCLC
TRQR7A81	25.6	28.3	2.7	25.3	30.9	5.6	24.4	29.6	5.2
2YHODAZN	28.6	36.8	8.2	24.2	31.8	7.6	23.5	31.1	7.6
QISIZA1M	25.4	33.8	8.3	24.4	32.0	7.7	23.1	31.5	8.3
AF8ALAN9	27.8	33.4	5.6	23.8	31.2	7.4	23.2	30.0	6.8
1173692B	28.2	36.5	8.4	24.2	34.0	9.8	23.2	32.1	9.0
1173127B	29.8	37.5	7.8	25.0	33.9	8.9	25.0	33.2	8.2
1173740B	32.0	37.9	5.9	24.4	32.4	8.0	23.3	31.9	8.5
Mean	28.2	34.9	6.7	24.4	32.3	7.9	23.7	31.3	7.7
SD	2.29	3.38	2.10	0.53	1.21	1.32	0.72	1.25	1.31
	Mean Ct value
	IPO8 (71 bp)			ABL1 (91 bp)			PUM1 (89 bp)
	Frozen NSCLC	FFPE NSCLC	ΔCt FFPE vs frozen NSCLC	Frozen NSCLC	FFPE NSCLC	ΔCt FFPE vs frozen NSCLC	Frozen NSCLC	FFPE NSCLC	ΔCt FFPE vs frozen NSCLC
FFPE NSCLC
TRQR7A81	24.6	29.3	4.6	23.9	28.9	5.1	22.5	28.3	5.8
2YHODAZN	24.4	31.5	7.0	23.7	32.4	8.7	20.9	30.1	9.1
QISIZA1M	23.6	31.1	7.5	22.6	31.7	9.1	21.4	31.2	9.8
AF8ALAN9	23.5	29.5	6.1	23.2	29.9	6.7	21.1	29.3	8.2
1173692B	23.9	31.6	7.7	23.8	33.1	9.3	21.5	32.0	10.5
1173127B	24.7	31.9	7.2	24.3	32.3	8.1	22.2	32.5	10.2
1173740B	24.1	30.9	6.8	24.2	33.7	9.5	21.6	32.1	10.4
Mean	24.1	30.8	6.7	23.7	31.7	8.1	21.6	30.8	9.2
SD	0.48	1.03	1.06	0.58	1.70	1.63	0.58	1.60	1.70

Abbreviations: Ct, cycle threshold; FFPE, formalin-fixed paraffin-embedded; NSCLC, non–small cell lung cancer.