Table 6.
Comparison of Ct values in FFPE NSCLC tissues and FFPE normal lung tissue for HRG plus 5 reference genes.
| Mean Ct values |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
|
HRG (72 bp) |
HMBS (64 bp) |
EIF2B1 (75 bp) |
|||||||
| FFPE NSCLC | FFPE normal lung | ΔCt FFPE NSCLC vs normal lung | FFPE NSCLC | FFPE normal lung | ΔCt FFPE NSCLC vs normal lung | FFPE NSCLC | FFPE normal lung | ΔCt FFPE NSCLC vs normal lung | |
| FFPE NSCLC | |||||||||
| TRQR7A81 | 28.3 | 34.3 | −6.0 | 30.9 | 32.9 | −2.0 | 29.6 | 31.0 | −1.4 |
| 2YHODAZN | 36.8 | 36.1 | 0.7 | 31.8 | 33.4 | −1.6 | 31.1 | 32.9 | −1.8 |
| QISIZA1M | 33.8 | 34.3 | −0.6 | 32.0 | 32.2 | −0.1 | 31.5 | 31.4 | 0.1 |
| AF8ALAN9 | 33.4 | 34.9 | −1.5 | 31.2 | 32.4 | −1.3 | 30.0 | 31.3 | −1.3 |
| 1173692B | 36.5 | 37.6 | −1.0 | 34.0 | 35.6 | −1.7 | 32.1 | 33.7 | −1.6 |
| FFPE normal lung | |||||||||
| 1171535B | NA | 35.1 | NA | NA | 35.5 | NA | NA | 32.6 | NA |
| 1173394B | NA | 29.0 | NA | NA | 29.8 | NA | NA | 27.6 | NA |
| 1173400B | NA | 29.2 | NA | NA | 29.7 | NA | NA | 27.6 | NA |
| Mean | 33.77 | 33.8 | −1.7 | 32.0 | 32.7 | −1.3 | 30.9 | 31.0 | −1.2 |
| SD | 3.41 | 3.10 | 2.55 | 1.21 | 2.21 | 0.72 | 1.06 | 2.29 | 0.75 |
| IPO8 (71 bp) | ABL1 (91 bp) | PUM1 (89 bp) | |||||||
| FFPE NSCLC | |||||||||
| TRQR7A81 | 29.3 | 30.2 | −2.0 | 28.9 | 30.5 | −1.6 | 28.3 | 30.4 | −2.1 |
| 2YHODAZN | 31.5 | 31.7 | −0.3 | 32.4 | 33.4 | −1.0 | 30.1 | 32.4 | −2.4 |
| IPO8 (71 bp) | ABL1 (91 bp) | PUM1 (89 bp) | |||||||
| QISIZA1M | 31.1 | 30.5 | 0.6 | 31.7 | 31.3 | 0.4 | 31.2 | 31.2 | 0.0 |
| AF8ALAN9 | 29.5 | 30.5 | −1.0 | 29.9 | 30.9 | −0.9 | 29.3 | 30.8 | −1.5 |
| 1173692B | 31.6 | 32.4 | −0.8 | 33.1 | 33.7 | −0.7 | 32.0 | 33.7 | −1.7 |
| FFPE Normal lung | |||||||||
| 1171535B | NA | 31.5 | NA | NA | 32.7 | NA | NA | 32.3 | NA |
| 1173394B | NA | 27.1 | NA | NA | 27.0 | NA | NA | 25.9 | NA |
| 1173400B | NA | 27.2 | NA | NA | 26.6 | NA | NA | 25.9 | NA |
| Mean | 30.6 | 30.2 | −0.5 | 31.2 | 30.8 | −0.8 | 30.2 | 30.3 | −1.5 |
| SD | 1.12 | 1.97 | 0.68 | 1.72 | 2.71 | 0.72 | 1.49 | 2.94 | 0.94 |
Abbreviations: Ct, cycle threshold; FFPE, formalin-fixed paraffin-embedded; HRG, heregulin; NA, not applicable (no matched tissue available); NSCLC, non–small cell lung cancer.