. 2017 Mar 30;12:1177271917699850. doi: 10.1177/1177271917699850

Table 7.

PCR efficiency and linearity for HRG and the 3 housekeeping genes (HMBS, EIF2B1, and IPO8).

Gene target	Run 1	Run 2	Run 3	Run 4	Run 5	Run 6	Mean
PCR efficiency, %
HRG	93.3^a	97.6^a	92.6^a	91.0^a	98.1^a	88.5^a	93.5^a
HMBS	94.8	93.3	91.2	97.8	99.0	102.3	96.4
EIF2B1	93.3	93.6	94.7	92.5	93.8	98.4	94.4
IPO8	100.0	101.0	99.1	99.8	96.1	99.9	99.3
R²
HRG	0.9966^a	0.9922^a	0.9986^a	0.9954^a	0.9958^a	0.9972^a	0.9960^a
HMBS	0.9988	0.9925	0.9925	0.9987	0.9986	0.9977	0.9965
EIF2B1	0.9987	0.9994	0.9988	0.9986	0.9982	0.9979	0.9986
IPO8	0.9992	0.9986	0.9989	0.9991	0.9989	0.9984	0.9989

Abbreviation: HRG, heregulin.

No amplification was seen in the HRG assay at 0.01 ng complementary DNA. The calculation was based on 7 data points instead of 8 data points.