Table 8.

Intra-run reproducibility: normalized Ct values for HRG (ΔCt) for each RNA extraction and FFPE sample and mean ΔCt deviation comparison.

Sample ID	Intra-run ΔCt								Intra-run mean ΔCt deviation comparisona
	Run 1	Run 2	Run 3	Run 4	Run 5	Run 6	Mean	SD	ΔCt highest	ΔCt lowest	X = highest − lowest	ΔCt deviation (2x)
FFPE NSCLC
1173692B	3.6	N/A	3.3	3.0	3.0	2.4	3.0	0.43	3.6	2.4	1.2	2.3
1173127B	5.5	4.3	4.7	3.6	4.2	4.1	4.4	0.65	5.5	3.6	2.0	3.8
1173740B	7.4	7.0	N/A	7.2	6.6	7.0	7.0	0.27	7.4	6.6	0.7	1.7
2YHODAZN	5.9	5.8	5.4	5.3	7.7	5.7	6.0	0.89	7.7	5.3	2.4	5.4
TRQR7A81	−1.6	−1.7	−1.5	−1.6	−1.4	−1.7	−1.6	0.11	−1.4	−1.7	0.3	1.2
FFPE normal lungb	3.3	3.0	3.1	3.0	3.4	3.1	3.2	0.16	3.4	3.0	0.4	1.3
Positive controlc	3.7	3.7	3.6	3.6	3.6	3.6	3.6	0.04	3.7	3.6	0.1	1.1

Abbreviations: Ct, cycle threshold; Ext., extraction (RNA); HRG, heregulin.

^aΔCt deviation calculations using highest and lowest HRG expression values for each patient extraction set.

^bTRQR7N58.

^cUniversal Human Reference RNA.