Table 9.

Inter-run reproducibility: normalized Ct values for HRG (ΔCt) for each run and FFPE sample and mean ΔCt deviation comparison.

Sample ID	Inter-run ΔCt							Inter-run mean ΔCt deviation comparisona
	Run 1	Run 2	Run 3	Run 4	Run 5	Mean	SD	ΔCt highest	ΔCt lowest	X = highest − lowest	ΔCt deviation (2x)
FFPE NSCLC
1173692B	3.5	3.8	3.7	4.2	3.6	3.7	0.27	4.2	3.5	0.7	1.7
1173127B	5.3	4.7	5.4	4.1	5.4	5.0	0.58	5.4	4.1	1.3	2.5
1173740B	6.6	7.9	7.1	6.9	6.6	7.0	0.53	7.9	6.6	1.3	2.5
2YHODAZN	5.4	5.5	5.2	6.0	6.2	5.7	0.43	6.2	5.2	1.0	2.0
TRQR7A81	−1.2	−1.2	−1.2	−1.3	−1.1	−1.2	0.06	−1.1	−1.3	0.2	1.1
FFPE normal lungb	3.0	2.9	3.0	2.8	3.1	3.0	0.09	3.1	2.8	0.2	1.2
Positive controlc	3.5	3.6	3.6	3.6	3.6	3.6	0.04	3.6	3.5	0.1	1.1

Abbreviations: Ct, cycle threshold; HRG, heregulin.

^aΔCt deviation calculations using highest and lowest HRG expression values for each patient extraction set.

^bTRQR7N58.

^cUniversal Human Reference RNA.