Skip to main content
. Author manuscript; available in PMC: 2018 Apr 5.
Published in final edited form as: Neuron. 2017 Apr 5;94(1):58–64.e3. doi: 10.1016/j.neuron.2017.03.018

Figure 3. Effect of domain swapping on open probability.

Figure 3

(A) The open channel blocker MK-801 (1 μM) inhibits steady state currents activated by 100 μM glycine and 1 mM glutamate from A/A, A/B and B/B receptors at different rates, consistent with distinct channel open probabilities. (B) MK-801 block of B/B receptors with a single ATD (Ba/B; magenta trace) or both ATDs (Ba/Ba; light blue trace) swapped to the GluN2A ATD. Red and blue traces indicate currents for A/A and B/B receptors, respectively (same in C and D). (C) MK-801 block of A/A receptors with a single ATD (A/Ab; magenta trace) or both ATDs (Ab/Ab; light blue trace) swapped to the GluN2B ATD. (D) MK-801 block of A/A receptors with a single LBD+TMD swapped to the GluN2B LBD+TMD (A/Ba; magenta trace) and B/B receptors with a single LBD+TMD swapped to the GluN2A LBD+TMD (Ab/B; light blue trace). (E) Bar graph with a summary of time constants for MK-801 block (τ). Data are mean ± SEM.