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. Author manuscript; available in PMC: 2017 Apr 14.
Published in final edited form as: J Neurosci Methods. 2017 Feb 13;280:64–76. doi: 10.1016/j.jneumeth.2017.02.002

Figure 1. EDTA, NaCl, and SDS concentrations significantly alter protein yield during solubilization of TRIzol-precipitated protein.

Figure 1

(A) Solubilization with standard lysis buffer (1mM EDTA, 140mM NaCl, 2% SDS, 10mM Tris) after TRIzol precipitation from three separate samples (each containing two combined rat hippocampus samples) yields on average 3.4μg/μL protein. (B) Varying the concentration of EDTA, NaCl, and SDS individually, while all other chemical components were held at standard lysis buffer levels, significantly influenced the protein yield during solubilization. Tris concentration did not impact yield. (C) Representative Coomassie stained gels of the data quantified in (B).