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. Author manuscript; available in PMC: 2017 Apr 14.
Published in final edited form as: J Neurosci Methods. 2017 Feb 13;280:64–76. doi: 10.1016/j.jneumeth.2017.02.002

Figure 3. Optimized lysis buffer solubilizes TRIzol-precipitated protein more effectively than commonly used buffers in a variety of time and temperature incubations.

Figure 3

(A) Optimized lysis buffer significantly increased protein yield during solubilization (30mins at 100°C) relative to other lysis buffers (RIPA, 1% SDS, and standard lysis buffer [see Fig. 1]). Protein yield via solubilization in optimized lysis buffer does not significantly differ when performed at a variety of temperatures (B) or for varying lengths of time (C). Representative Coomassie stained gels of the data quantified are depicted in A′–C′.